Treatment of human spermatozoa with an egg yolk medium can enhance the outcome of in vitro fertilization

In at least 4 of 7 cases, fertilization of intact human oocytes was more successful when spermatozoa were pretreated with TEST yolk medium at 5 degrees C for 2 hours as compared with the standard treatment with Ham's F-10 only. Both pregnancies that were obtained after the transfer of the ferti...

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Published inFertility and sterility Vol. 52; no. 6; pp. 1077 - 1079
Main Authors Katayama, K.Paul, Stehlik, Edward, Roesler, Mark, Jeyendran, Rajasingam S., Holmgren, Wendy J., Zaneveld, Lourens J.D.
Format Journal Article
LanguageEnglish
Published New York, NY Elsevier Inc 01.12.1989
Elsevier Science
Subjects
Biological and medical sciences
Birth control
Culture Media
Egg Yolk
Fertilization in Vitro
Gynecology. Andrology. Obstetrics
Humans
Male
Medical sciences
Spermatozoa - drug effects
Sterility. Assisted procreation
Human
Culture medium
Spermatozoa
Fertilizing ability
Zona-free ova penetration test
Assisted procreation
Fecundation
In vitro
Comparative study
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Summary:In at least 4 of 7 cases, fertilization of intact human oocytes was more successful when spermatozoa were pretreated with TEST yolk medium at 5 degrees C for 2 hours as compared with the standard treatment with Ham's F-10 only. Both pregnancies that were obtained after the transfer of the fertilized oocytes resulted from oocytes fertilized by TEST yolk-treated spermatozoa. No decrease in fertilization occurred in any of the cases after TEST yolk treatment. If these results hold true for a larger series of patients, it may be worthwhile for the standard IVF incubation system of spermatozoa to include TEST yolk.
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ISSN:0015-0282
1556-5653
DOI:10.1016/S0015-0282(16)53181-1