Depolymerization of legume seed galactomannan by Celloviridin G20x

• Published in: Applied biochemistry and microbiology Vol. 42; no. 5; pp. 508 - 513
• Main Authors: Ilyina, A V, Mestechkina, N M, Shcherbukhin, V D, Varlamov, V P
• Format: Journal Article
• Language: English
• Published: Dordrecht Springer Nature B.V 01.09.2006
• Subjects: Cyamopsis tetragonoloba; Galega; Gleditsia triacanthos; Molecular weight; Sophora
• ISSN: 0003-6838; 1608-3024
• DOI: 10.1134/S0003683806050115

The depolymerization of legume galactomannans by the commercial preparation Celloviridin G20x was studied with the aim of obtaining macromolecular fragments of a constant composition. Four galactomannans, representative of the entire range of monomer ratios characteristic of this class of phytopolysaccharides, were hydrolyzed. The galactomannans were isolated from oriental goat's rue (Galega orientalis Lam., Man: Gal 1.1), guar (Cyamopsis tetragonoloba L., Man: Gal 1.6), honeylocust (Gleditsia triacanthos f. inermis L., Man: Gal 2.4), and sophora (Styphnolobium japonicum (L.) Schott., Man: Gal 5.1). Fragments with a monomer ratio close to that in the original polysaccharides were obtained with a high yield (75-80%). The degree of substitution of the 1,4-β-D-mannopyranose chain at position C-6 with α-galactose residues influenced the molecular weight of the final reaction product.[PUBLICATION ABSTRACT]