Determination of benzhexol hydrochloride by capillary zone electrophoresis with an end-column electrochemiluminescence detection

• Published in: Microchemical journal Vol. 76; no. 1; pp. 11 - 16
• Main Authors: Yan, Jilin, Liu, Jifeng, Cao, Weidong, Sun, Xiuhua, Yang, Xiurong, Wang, Erkang
• Format: Journal Article
• Language: English
• Published: Elsevier B.V 01.02.2004
• Subjects: Benzhexol hydrochloride; Capillary electrophoresis; Electrochemiluminescence; Capillary electrophoresis; Electrochemiluminescence; Benzhexol hydrochloride
• ISSN: 0026-265X; 1095-9149
• DOI: 10.1016/j.microc.2003.10.003

A capillary zone electrophoresis with end-column electrochemiluminescence (ECL) detector was described for the determination of benzhexol hydrochloride. The detection was based on the tris(2,2′-bypyridine)ruthenium(II) [Ru(bpy) 3 2+] ECL reaction with the analyte. Electrophoresis was performed using a 25 μm i.d. uncoated capillary. 10 mM sodium phosphate buffer (pH=8.0) was used as the running buffer. The solution in the detection cell was 80 mM sodium phosphate (pH=8.0) and 5 mM Ru(bpy) 3 2+. A linear calibration curve of three-orders of magnitude was obtained (with a correlation coefficient of >0.999) from 1.0×10 −8 to 1.0×10 −5 M and the limit of detection was 6.7×10 −9 M ( S/ N=3). This just provides an easy and sensitive method to determine the active ingredient in pharmaceutical formulations.