Influence of buffer electrolyte pH on the migration behavior of phenolic compounds in co-electroosmotic capillary electrophoresis

• Published in: Journal of Chromatography A Vol. 693; no. 2; pp. 359 - 365
• Main Authors: Masselter, Sonja M., Zemann, Andreas J.
• Format: Journal Article
• Language: English
• Published: Elsevier B.V 24.02.1995
• ISSN: 0021-9673
• DOI: 10.1016/0021-9673(94)01112-R

The separation behavior of phenolic compounds with various substituents was investigated with co-electroosmotic capillary electrophoresis (migration of the analytes in the same direction as the electroosmotic flow). Co-electroosmotic flow conditions were set up when the electroosmotic flow direction was reversed by adding either a cationic surfactant (cetyltrimethylammonium bromide) or a polycation (1,5-dimethyl-1,5-diazaundecamethylene polymethobromide) to the buffer. An alkaline buffer electrolyte was chosen to ensure complete dissociation of the phenols (pH 10–12). It was shown that a separation of the chosen phenols was possible under co-electroosmotic conditions by optimizing the buffer pH.