Modeling and Optimization of Chromatographic Purification of Arglabin from CO2 Extract of Artemisia glabella Kar. et Kir

• Published in: Chromatographia Vol. 84; no. 11; pp. 1077 - 1086
• Main Authors: Adekenov, S. M., Khabarov, I. A., Yakovenko, G. A., Adekenova, A. S., Berthod, A.
• Format: Journal Article
• Language: English
• Published: Berlin/Heidelberg Springer Berlin Heidelberg 01.11.2021; Springer Nature B.V; Springer Verlag
• Subjects: Acetonitrile; Analytical Chemistry; Biochemistry, Molecular Biology; Carbon dioxide; Chemical Sciences; Chemistry; Chemistry and Materials Science; Chromatography; Design factors; Design optimization; Ethyl acetate; Flow velocity; Heptanes; Laboratory Medicine; Life Sciences; Original; Pharmacy; Productivity; Proteomics; Purification; Supercritical gas extraction; Productivity; Arglabin; Countercurrent chromatography; Preparative purification; Centrifugal partition chromatography; Optimization
• ISSN: 0009-5893; 1612-1112
• DOI: 10.1007/s10337-021-04093-0

The dried aerial parts of the plant Artemisia glabella Kar. et Kir. contain about 1.1% of the very important sesquiterpene lactone arglabin. This compound can be extracted by supercritical CO 2 giving a 20 time concentrated extract containing about 22% arglabin. The CO 2 extract can be efficiently treated by hydrostatic countercurrent chromatography (CCC) able to produce in a single run 95% pure arglabin. Both mobile and stationary phase are liquid in CCC. The biphasic waterless liquid system heptane:ethyl acetate:acetonitrile 2:1:2 v/v/v was used to separate arglabin from its two major co-extracted compounds: argolide and eucalyptol. A three-factor Box–Behnken design was used to optimize the CCC preparative purification of the CO 2 extract by a FCPC-5L instrument. Injected amount, mobile phase flow rate, and rotor rotation speed were used to optimize arglabin productivity. An optimized run was able to treat 90 g of extract producing 20 g of 95% pure arglabin in 50 min, a theoretical productivity of 400 mg/min.