A rapid, quantitative, and selective estimation of radioactively labeled peptidoglycan in gram-positive bacteria

The Park and Hancock procedure for the isolation of bacterial cell wall peptidoglycans has been modified. The method developed permits rapid and accurate determination of small quantities of radioactively labeled material. The principal modifications consist of carrying out the procedure on glass-fi...

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Bibliographic Details
Published inAnalytical biochemistry Vol. 44; no. 2; pp. 645 - 653
Main Authors Boothby, D., Daneo-Moore, L., Shockman, G.D.
Format Journal Article
LanguageEnglish
Published Elsevier Inc 01.01.1971
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Summary:The Park and Hancock procedure for the isolation of bacterial cell wall peptidoglycans has been modified. The method developed permits rapid and accurate determination of small quantities of radioactively labeled material. The principal modifications consist of carrying out the procedure on glass-fiber filters after a vigorous hydrolysis of the cells with TCA (96°C, 30 min) and using Pronase in place of trypsin.
ISSN:0003-2697
1096-0309
DOI:10.1016/0003-2697(71)90255-7