Glycine-extended gastrin potentiates gastrin-stimulated gastric acid secretion in rats

• Published in: The American journal of physiology Vol. 270; no. 1 Pt 1; p. G220
• Main Authors: Higashide, S, Gomez, G, Greeley, Jr, G H, Townsend, Jr, C M, Thompson, J C
• Format: Journal Article
• Language: English
• Published: United States 01.01.1996
• Subjects: Animals; Drug Synergism; Gastric Acid - secretion; Gastrins - pharmacology; Histamine - pharmacology; Hormones - pharmacology; Injections, Intravenous; Male; Rats; Rats, Inbred F344
• ISSN: 0002-9513
• DOI: 10.1152/ajpgi.1996.270.1.g220

The purpose of this study was to examine whether an intermediate form of amidated gastrin, glycine-extended gastrin (Gly-G), can stimulate gastric acid secretion in conscious rats prepared with gastric fistulas. Intravenous administration of Gly-G (20 nmol.kg-1.h-1) alone for 2 h did not stimulate gastric acid secretion; however, administration of Gly-G (20 nmol.kg-1.h-1) in combination with a bolus administration of gastrin (9.5 nmol/kg) potentiated acid secretion significantly. Gastric acid secretion in response to gastrin alone and gastrin plus Gly-G (2 nmol.kg-1.h-1) was 109.1 +/- 21.6 and 170.1 +/- 27.7 mueq.kg-1.h-1, respectively (P < 0.05). Gastric acid secretion in response to gastrin alone and gastrin plus Gly-G (20 nmol.kg-1.h-1) was 84.8 +/- 17.5 and 164.1 +/- 29.3 mueq.kg-1.h-1, respectively (P < 0.05). Intravenous administration of Gly-G (20 nmol.kg-1.h-1) failed to increase histamine (1 mg/kg)-stimulated acid output. These results demonstrate that Gly-G can selectively potentiate the stimulatory effect of gastrin on acid secretion in rats and that the unprocessed form of gastrin, Gly-G, can exert a biological effect in the stomach.