Effect of various glucagon/insulin molar ratios on blood ketone body levels in rats by use of osmotic minipumps

• Published in: Diabetes research and clinical practice Vol. 34; no. 1; pp. 1 - 6
• Main Authors: Ubukata, Eiichi, Mokuda, Osamu, Sakamoto, Yoshikazu, Shimizu, Naokata
• Format: Journal Article
• Language: English
• Published: Shannon Elsevier Ireland Ltd 01.09.1996; Elsevier Science
• Subjects: 3-Hydroxybutyric Acid; Acetoacetates - blood; Animals; Biological and medical sciences; Diabetes. Impaired glucose tolerance; Drug Interactions; Endocrine pancreas. Apud cells (diseases); Endocrinopathies; Etiopathogenesis. Screening. Investigations. Target tissue resistance; Fatty Acids, Nonesterified - blood; Glucagon - administration & dosage; Glucagon - pharmacology; Glucagon/insulin molar ratio; Hydroxybutyrates - blood; Infusions, Parenteral; Insulin - administration & dosage; Insulin - pharmacology; Ketone Bodies - blood; Ketone body; Male; Medical sciences; Osmotic minipump; Rats; Rats, Wistar; Time Factors; Ketone body; Osmotic minipump; Glucagon/insulin molar ratio; Endocrinopathy; Pancreatic hormone; Glucagon; Rat; Diabetes mellitus; Peptide hormone; Rodentia; Metabolism; Insulin; Protein hormone; Vertebrata; Mammalia; Animal; Hormonal regulation; Complication; Ratio; Hormonal investigation; Osmotic pump
• ISSN: 0168-8227; 1872-8227
• DOI: 10.1016/S0168-8227(96)01325-3

The bihormonal control by insulin and glucagon of blood ketone body level was studied. Mixed solutions with various molar ratios of glucagon and insulin ( G I ) were subcutaneously infused continuously for five days by use of the osmotic minipump in the normal rats. The concentrations of insulin and glucagon solution were set at the high G I molar ratio, the moderate G I molar ratio and the low G I molar ratio. In addition, the moderate G I molar ratio group was divided into three sub-groups: low glucagon and low insulin, moderate glucagon and moderate insulin, and high glucagon and high insulin. After five days, the rats were decapitated to measure plasma ketone body, free fatty acid (FFA), glucose, insulin and glucagon. The FFA level was not significantly different among three groups. The glucose level was not different between the high and moderate G I molar ratio groups, and decreased in the low G I molar ratio group. 3-β-hydroxybutyrate (3-OHBA) and acetoacetate (AcAc) levels in the high G I molar ratio group were elevated, and 3-OHBA level in the low G I molar ratio group was lowered compared to those in the moderate G I molar ratio group. Among three moderate G I molar ratio sub-groups, there was no difference in 3-OHBA and AcAc levels. These results demonstrate that plasma ketone body levels are controlled by the plasma G I molar ratio.