Spermatogenesis in vitro: completion of meiosis and early spermiogenesis

In vitro formation of haploid spermatids has not been convincingly demonstrated in mammals. To investigate this problem we selected defined segments of rat seminiferous tubules containing late pachytene and diakinetic primary spermatocytes (Stages XII and XIII of the cycle) for culture in a chemical...

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Published inEndocrinology (Philadelphia) Vol. 112; no. 3; p. 1150
Main Authors Parvinen, M, Wright, W W, Phillips, D M, Mather, J P, Musto, N A, Bardin, C W
Format Journal Article
LanguageEnglish
Published United States 01.03.1983
Subjects
Animals
Cell Differentiation
Cells, Cultured
Male
Meiosis
Microscopy, Electron
Rats
Seminiferous Tubules - physiology
Spermatids - physiology
Spermatids - ultrastructure
Spermatocytes - physiology
Spermatogenesis
Spermatozoa - physiology
Testis - physiology
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Summary:In vitro formation of haploid spermatids has not been convincingly demonstrated in mammals. To investigate this problem we selected defined segments of rat seminiferous tubules containing late pachytene and diakinetic primary spermatocytes (Stages XII and XIII of the cycle) for culture in a chemically defined medium. After 2 days, most spermatocytes completed both meiotic divisions, and by 6 days the tubular epithelium developed morphologic characteristics of Stage V in which the newly formed spermatids had acrosomic systems characteristic of step 5 spermiogenesis. The seminiferous tubules also differentiated biochemically as evidenced by increased production of proteins characteristically secreted by Stage V. Since this in vitro differentiation of the germinal epithelium occurred in the absence of testosterone and FSH, we conclude that late pachytene spermatocytes and their associated Sertoli cells have all the information required for both meiotic divisions and early spermiogenesis.
ISSN:0013-7227
DOI:10.1210/endo-112-3-1150