Monolayers of photosynthetic reaction centers of green and purple bacteria

Monolayers of the reaction centers isolated from the photosynthetic bacteria Chloroflexus aurantiacus, Rhodobacter sphaeroides and Rhodopseudomonas viridis were prepared and investigated. The monolayer characteristics of these proteins were analyzed to determine the optimum conditions for stable fil...

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Published inThin solid films Vol. 210; pp. 723 - 725
Main Authors Zaitsev, S.Yu, Kalabina, N.A., Zubov, V.P., Lukashev, E.P., Kononenko, A.A., Uphaus, R.A.
Format Journal Article
LanguageEnglish
Published Elsevier B.V 30.04.1992
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Summary:Monolayers of the reaction centers isolated from the photosynthetic bacteria Chloroflexus aurantiacus, Rhodobacter sphaeroides and Rhodopseudomonas viridis were prepared and investigated. The monolayer characteristics of these proteins were analyzed to determine the optimum conditions for stable film preparations. Monolayers were transfered onto quartz and metal supports by the Langmuir-Schaefer method to provide multilayers of definite thickness. These films retain the optical and photoelectrical properties of the native photosynthetic membranes. The sign of the photopotential provides evidence for a different orientation of the reaction centers of Rb. sphaeroides compared to those of Rps. viridis.
ISSN:0040-6090
1879-2731
DOI:10.1016/0040-6090(92)90385-O