Application of triple potential step amperometry method for quantitative electroanalysis

• Published in: Chinese science bulletin Vol. 52; no. 20; pp. 2771 - 2774
• Main Authors: Liu, ChenMing, Cao, HongBin, Li, YuPing, Zhang, Yi
• Format: Journal Article
• Language: English
• Published: Institute of Process Engineering, Chinese Academy of Sciences, Beijing 100080, China 01.10.2007; Graduate University of Chinese Academy of Sciences, Beijing 100049, China%Institute of Process Engineering, Chinese Academy of Sciences, Beijing 100080, China
• Subjects: 电子学; 电流测定法; 电量电解; 电阻板; nitroreductase; electroanalysis; enzyme-modified electrode; triple potential step amperometry
• ISSN: 1001-6538; 1861-9541
• DOI: 10.1007/s11434-007-0379-6

A novel quantitative electroanalysis method, triple potential step amperometry （TPSA）, was developed and explained with an example of nitrobenzene analyzing in water. The selectivity of TPSA was improved by controlling the potential step within a narrow interval and using enzyme-modified electrode, the narrow potential step makes the method avoid most interferents, and enzyme-modified electrode can enhance the response of target substance selectively. The peak area was investigated for quantitative calibration, such as nitrobenzene concentration showing a linear relation with the peak area, with the correlation coefficients being 0.9995. The t-test and F-test were applied to evaluating the reliability of TPSA, the results showed that there was no evidence of systematic error for TPSA, and the method was of no significant difference from CV. The merit of fast detecting and few potential changing times make the TPSA suitably applicable to low-cost automatic monitoring equipments.