Study of galactoglucomannans from the seed of Cercis canadensis L

• Published in: Applied biochemistry and microbiology Vol. 41; no. 3; pp. 283 - 288
• Main Authors: Mestechkina, N M, Egorov, A V, Anulov, O V, Shcherbukhin, V D
• Format: Journal Article
• Language: English
• Published: Dordrecht Springer Nature B.V 01.05.2005
• Subjects: Cercis canadensis; Fabaceae; Spectrum analysis
• ISSN: 0003-6838; 1608-3024
• DOI: 10.1007/s10438-005-0048-y

Galactoglucomannans were isolated by selective precipitation from aqueous and alkaline extracts of endosperm and hulls of Cercis canadensis, a member of the family Fabaceae. Their monosaccharide composition (Man : Gal : Glu) was as follows: 10.4 : 0.9 : 1 (polysaccharide from the endosperm) and 4.5 : 0.9 : 1 (polysaccharide from the hulls). The identity of IR spectra was indicative of the similarity of their structure. Analysis of the structure of the galactoglucomannan from endosperm by ^sup 13^C NMR spectroscopy showed that its main chain consisted of 1,4-β-D-manno- and 1,4-β-D-glucopyranose. Part of the mannose residues in the chain were substituted at C6 with single residues of α-D-galactopyranose. Galactoglucomannans are located in different parts of the seed and implement different functions.[PUBLICATION ABSTRACT]