A rapid procedure for the isolation of RNA from high-phenolic-containing tissues of pecan

• Published in: HortScience Vol. 27; no. 12; pp. 1316 - 1318
• Main Authors: Levi, A, Galau, G.A, Wetzstein, H.Y
• Format: Journal Article
• Language: English
• Published: Alexandria, VA American Society for Horticultural Science 01.12.1992
• Subjects: Agronomy. Soil science and plant productions; ARN; Biological and medical sciences; BIOTECHNOLOGIE; Biotechnology; BIOTECNOLOGIA; CARYA PECAN; COMPOSE PHENOLIQUE; COMPUESTOS FENOLICOS; EMBRIONES VEGETALES; EMBRYON VEGETAL; FEUILLE; Fundamental and applied biological sciences. Psychology; Genetic engineering applications; Genetics and breeding of economic plants; HOJAS; METHODE; METODOS; Plant breeding: fundamental aspects and methodology; TEJIDOS VEGETALES; TISSU VEGETAL; Plant tissue; High content; Embryo; Biochemical analysis; RNA; Biochemical compound; Plant leaf; Solvent extraction; Polyphenol; Rapid technique; Analysis method; Juglandaceae; Carya illinoensis; Dicotyledones; Preparation; Intercompound effect; Angiospermae; Procedure; Isolation; Spermatophyta; Fruit tree; Technique; Nucleic acid
• ISSN: 0018-5345; 2327-9834
• DOI: 10.21273/HORTSCI.27.12.1316

A simple and efficient protocol is reported for the isolation of RNA from embryos and leaves of pecan [Carya illinoinensis (Wangenh.) K. Koch]. The method relies on suppression of the polyphenols from interaction with the RNA and their rapid removal from the homogenate by chloroforin extraction. This method produced abundant amounts of high-quality RNA. This protocol is likely to be useful for Juglandaceous species and other recalcitrant plants with high levels of phenolic compounds