Structure of solid-supported lipid–DNA–metal complexes investigated by energy dispersive X-ray diffraction

When mixing aqueous solutions of DNA and dioloeyl-phosphatidylcholine multilamellar liposomes in the presence of Mn 2+ ions, a multilamellar liquid-crystalline phase is formed in which DNA monolayers are comprised between opposing lipid bilayers. Here, we report on a new experimental procedure resul...

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Bibliographic Details
Published inChemical physics letters Vol. 397; no. 1; pp. 138 - 143
Main Authors Caracciolo, G., Sadun, C., Caminiti, R., Pisani, M., Bruni, P., Francescangeli, O.
Format Journal Article
LanguageEnglish
Published Elsevier B.V 11.10.2004
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Summary:When mixing aqueous solutions of DNA and dioloeyl-phosphatidylcholine multilamellar liposomes in the presence of Mn 2+ ions, a multilamellar liquid-crystalline phase is formed in which DNA monolayers are comprised between opposing lipid bilayers. Here, we report on a new experimental procedure resulting in the formation of solid-supported DOPC–DNA–Mn 2+ complexes in the biologically relevant excess water condition. The supramolecular structure of solid-supported complexes, characterized by means of Energy Dispersive X-ray Diffraction, exhibits very similar structural properties with respect to the solution structure of the complexes as revealed by synchrotron Small Angle X-ray Scattering measurements.
ISSN:0009-2614
1873-4448
DOI:10.1016/j.cplett.2004.08.102