Methoxyresorufin as a substrate for the fluorometric assay of insect microsomal O-dealkylases
Methoxy-, ethoxy-, propoxy-, and butoxyresorufin were prepared and tested as substrates for the fluorometric assay of O-dealkylation by the mixed-function oxidase system in house flies, Musca domestica L. Methoxyresorufin proved to be the most suitable substrate because of the favorable reaction rat...
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Published in | Pesticide biochemistry and physiology Vol. 7; no. 4; pp. 349 - 354 |
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Main Authors | , , , |
Format | Journal Article |
Language | English |
Published |
Elsevier Inc
01.01.1977
|
Online Access | Get full text |
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Summary: | Methoxy-, ethoxy-, propoxy-, and butoxyresorufin were prepared and tested as substrates for the fluorometric assay of
O-dealkylation by the mixed-function oxidase system in house flies,
Musca domestica L. Methoxyresorufin proved to be the most suitable substrate because of the favorable reaction rates. This sensitive assay can be performed with a minimum of microsomal protein (<0.3 mg/ml) in 5 min or less. The apparent
K
m
and maximum velocity were calculated as 2.88 μ
M and 0.27 nmol of resorufin produced/min/mg of microsomal protein, respectively. The
O-dealkylation reaction required O
2 and NADPH and was inhibited by CO. |
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ISSN: | 0048-3575 1095-9939 |
DOI: | 10.1016/0048-3575(77)90038-4 |