Early effects of carcinogenic aminoazo dyes on the protein patterns and metabolism in rat liver

• Published in: International journal of biochemistry Vol. 7; no. 6; pp. 259 - 270
• Main Authors: Yoshida, Michiteru, Holoubek, Viktor
• Format: Journal Article
• Language: English
• Published: Elsevier B.V 1976
• ISSN: 0020-711X
• DOI: 10.1016/0020-711X(76)90084-7

1. 1. The feeding of the carcinogenic 3′-methyl-4-dimethylaminoazobenzene (3′-Me-DAB) resulted in a pronounced stimulation of the incorporation of 14C amino acids into nearly all fractions of nuclear proteins of rat liver cells with one exception. The incorporation was suppressed in the fraction which contains the proteins of the nuclear ribonucleoprotein particles which are involved in the processing of newly synthesized nuclear RNA. 2. 2. Only limited quantitative changes were observed in the electrophoretic patterns of liver nuclear proteins of animals fed 3′-Me-DAB. 3. 3. In the cytoplasm the feeding of 3′-Me-DAB induced high increase in the amount of glutathione-S-transferase and of a specific microsomal hemoprotein. A smaller increase in the amounts of both proteins was observed also in animals fed non-carcinogenic 4-aminoazobenzene. 4. 4. The induced microsomal hemoprotein differed in mol. wt from the microsomal hemoproteins induced by phenobarbital and by 3-methylcholanthrene. 5. 5. Increased glutathione-S-transferase was identified to be identical with the minor cytoplasmic binder of 3′-Me-DAB.