Gene expression analysis of neural cells and tissues using DNA microarrays

DNA microarrays pose specific challenges to those studying the central and peripheral nervous systems. Probably the most important involve difficulty in obtaining appropriate tissue for study, as well as the problems posed by cellular heterogeneity. This unit describes advances in the available tech...

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Bibliographic Details
Published inCurrent protocols in neuroscience Vol. Chapter 4; p. Unit 4.28
Main Authors Karsten, Stanislav L, Kudo, Lili C, Geschwind, Daniel H
Format Journal Article
LanguageEnglish
Published United States 01.10.2008
Subjects
Animals
Flow Cytometry - methods
Flow Cytometry - standards
Gene Expression
Gene Expression Profiling - methods
Gene Expression Profiling - standards
Humans
Microdissection - methods
Microdissection - standards
Nerve Tissue Proteins - biosynthesis
Nerve Tissue Proteins - genetics
Nervous System - metabolism
Oligonucleotide Array Sequence Analysis - methods
Oligonucleotide Array Sequence Analysis - standards
Polymerase Chain Reaction - methods
Polymerase Chain Reaction - standards
RNA, Messenger - analysis
RNA, Messenger - genetics
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Summary:DNA microarrays pose specific challenges to those studying the central and peripheral nervous systems. Probably the most important involve difficulty in obtaining appropriate tissue for study, as well as the problems posed by cellular heterogeneity. This unit describes advances in the available technologies and provides protocols for cDNA microarray hybridization, including the use of PCR amplicons. Protocols are also provided for the two major methods for limiting cellular heterogeneity by study of RNA from single cell populations in high-throughput microarray studies, laser capture microdissection (LCM), and automated fluorescent cell sorting (FACS-array).
ISSN:1934-8576
DOI:10.1002/0471142301.ns0428s45