Pile burns as a proxy for high severity wildfire impacts on soil microbiomes

• Published in: Geoderma Vol. 448; p. 116982
• Main Authors: Fowler, Julie A., Nelson, Amelia R., Bechtold, Emily K., Paul, Raegan, Wettengel, Alexandra M., McNorvell, Michael A., Stevens-Rumann, Camille S., Fegel, Timothy S., Anderson, Erik, Rhoades, Charles C., Wilkins, Michael J.
• Format: Journal Article
• Language: English
• Published: Elsevier B.V 01.08.2024; Elsevier
• Subjects: Ectomycorrhizal fungi; Forest disturbance; High severity wildfires; Mesocosm; Microbial traits; Pile burns; Pyrophilous microbes; Soil microbiome; Wildfire proxy; LWL; PyOM; Wildfire proxy; Mesocosm; CSR; ASV; LWR; Pile burns; Microbial traits; LDA; NMDS; USFS; High severity wildfires; LEfSe; Soil microbiome; DOC; AMF; Pyrophilous microbes; Forest disturbance; EMF; Ectomycorrhizal fungi
• ISSN: 0016-7061; 1872-6259
• DOI: 10.1016/j.geoderma.2024.116982

•Soil microbial alpha and beta diversity decreased following pile burning.•Presence/type of burn scar structures soil microbiomes alongside location and time since burn.•Pile burns selected for pyrophilous taxa with increased 16S rRNA gene copy numbers.•High intensity pile burns represent a wildfire proxy for soil microbiome research.•Pile burns may clarify post-fire ectomycorrhizal loss impacts on forest recovery. Wildfires in the western US are increasing in frequency, size, and severity. These disturbances alter soil microbiome structure and function, with greater fire severity leading to more pronounced impacts to bacterial, archaeal, and fungal communities. These changes have implications for the provisioning of microbially-mediated ecosystem services (e.g., carbon sequestration, clean water supplies) typically associated with forested watersheds. Challenges in sampling wildfire-impacted areas immediately post-burn have limited our assessment of short-term (i.e., days to weeks) changes in the soil microbiome and understanding of how microbial populations may influence post-fire biogeochemistry and ecosystem recovery. The identification of potential high severity wildfire proxies may help address some of these knowledge gaps. One potential proxy is pile burns scars, which are produced from a set of common techniques for fuel disposal and site preparation in conifer forests throughout the western US and beyond. We sampled depth-resolved layers from fire-impacted soil and combusted litter and woody materials in a series of recent pile burn scars near West Yellowstone, Montana and nearby unburned mineral soil controls to assess whether the pile burn scars exhibited microbial signatures characteristic of forest soils impacted by recent high severity wildfire. Changes in soil carbon and nitrogen chemistry and patterns of microbial alpha and beta diversity broadly aligned with those observed following wildfire, particularly the enrichment of so-called ‘pyrophilous’ taxa. Furthermore, many of the taxa enriched in burned soils likely encoded putative traits that benefit microorganisms colonizing these environments, such as the potential for fast growth or utilization of pyrogenic carbon substrates. We suggest that pile burn scars may represent a useful proxy along the experimental gradient from muffle furnace or pyrocosm studies to largescale prescribed burns in the field to advance understanding of the soil (and related layers, like ash) microbiome following high severity wildfires, particularly when coupled with experimental manipulation. Finally, we discuss existing research gaps that experimentally manipulated pile burns could be utilized to address.