Linkers, resins, and general procedures for solid-phase peptide synthesis

• Published in: Methods in molecular biology (Clifton, N.J.) Vol. 1047; p. 23
• Main Authors: Shelton, Pernille Tofteng, Jensen, Knud J
• Format: Journal Article
• Language: English
• Published: United States 01.01.2013
• Subjects: Cross-Linking Reagents - chemistry; Peptides - chemical synthesis; Peptides - chemistry; Resins, Synthetic - chemistry; Solid-Phase Synthesis Techniques
• ISSN: 1940-6029
• DOI: 10.1007/978-1-62703-544-6_2

This chapter describes the basic protocols for solid-phase peptide synthesis using the Fmoc group as the N (α)-protecting group (Fmoc-SPPS). The chapter introduces resins and their handling, choice of linkers, and the most common methods for peptide chain assembly. The proper choice of resins and linkers for solid-phase synthesis is a key parameter for successful peptide synthesis. This chapter provides an overview of the most common and useful resins and linkers for the synthesis of peptides with C-terminal amides, carboxylic acids, and more. The chapter finishes with robust protocols for general solid-phase peptide synthesis, i.e., the standard operations.