A novel multilayer hydrogel wound dressing for antibiotic release

• Published in: Journal of drug delivery science and technology Vol. 58; p. 101536
• Main Authors: Tamahkar, Emel, Özkahraman, Bengi, Süloğlu, Aysun Kılıç, İdil, Neslihan, Perçin, Işık
• Format: Journal Article
• Language: English
• Published: Elsevier B.V 01.08.2020
• Subjects: Antibacterial activity; Antibiotic release; Multilayer hydrogels; Wound dressing; Antibacterial activity; Multilayer hydrogels; Wound dressing; Antibiotic release
• ISSN: 1773-2247
• DOI: 10.1016/j.jddst.2020.101536

In this study, the novel multilayer (ML) hydrogels were developed as antibacterial wound dressings. ML hydrogels were prepared as four layers using carboxylated polyvinyl alcohol (PVA-C), gelatin (G), hyaluronic acid (HA) and gelatin respectively. The upper layers (PVA-C and G) provide the moist control and physical barrier for microorganisms. The HA based middle layer was designed as an antibiotic-loaded layer. The lower layer serves as the controlling membrane for antibiotic release and provides the removal of excess exudate from the wound site. The ML hydrogels were characterized with FT-IR, SEM, DSC, swelling tests and hydrolytic degradation tests. Cell viability assay was also performed in L929 fibroblast cells in vitro. The in-vitro drug release profile of ML hydrogels was investigated at pH 7.4 at 37 °C and it was found that 63% of the antibiotic was released after 7 days. [Display omitted] •Multilayer (ML) hydrogels having four layers consisted of carboxylated-PVA, gelatin and hyaluronic acid were developed.•ML hydrogels with the controlled antibiotic release prolonged for 5 days present great potential as novel wound dressings.•ML hydrogels showed no cytotoxic effect to human fibroblasts.•ML hydrogels demonstrated antibacterial activity against oxacillin sensitive S. Aeurus.