DNA fragmentation in Marchantia polymorpha thalli in response to spermine treatment

• Published in: International journal of plant sciences Vol. 166; no. 4; pp. 589 - 594
• Main Authors: Pagoria, D.A, Maravolo, N.C
• Format: Journal Article
• Language: English
• Published: Chicago The University of Chicago Press 01.07.2005; University of Chicago, acting through its Press
• Subjects: apical meristems; apoptosis; Botany; Deoxyribonucleic acid; DNA; DNA fragmentation; epidermis (plant); Gene expression; Marchantia polymorpha; mesophyll; mosses and liverworts; plant development; plant genetics; Plant growth; plant response; spermine; Marchantia polymorpha; programmed cell death; polyamines; DNA fragmentation
• ISSN: 1058-5893; 1537-5315
• DOI: 10.1086/430193

Terminal deoxynucleotidyltransferase (TdT)-mediated dUTP nick end labeling (TUNEL) was used to identify internucleosomal DNA fragmentation in the liverwort Marchantia polymorpha. Fluorescence microscopy revealed that DNA fragmentation was localized near the lower epidermis of the apical meristem and progressed into mesophyll cells as a function of distance from the thallus apex. Plants treated with 100 μM spermine displayed significantly ( \documentclass{aastex} \usepackage{amsbsy} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{bm} \usepackage{mathrsfs} \usepackage{pifont} \usepackage{stmaryrd} \usepackage{textcomp} \usepackage{portland,xspace} \usepackage{amsmath,amsxtra} \usepackage[OT2,OT1]{fontenc} \newcommand\cyr{ \renewcommand\rmdefault{wncyr} \renewcommand\sfdefault{wncyss} \renewcommand\encodingdefault{OT2} \normalfont \selectfont} \DeclareTextFontCommand{\textcyr}{\cyr} \pagestyle{empty} \DeclareMathSizes{10}{9}{7}{6} \begin{document} \landscape $$P< 0.001$$ \end{document} ) lower levels of DNA fragmentation within aged (2-5 cm) tissue in comparison with untreated thalli. This study shows that exogenously applied spermine treatment significantly delays the onset of DNA fragmentation associated with senescing liverwort tissue.