Polydatin Alleviates Lipopolysaccharide-Triggered Inflammatory Injury Through Up-Regulating miR-125b in Chondrogenic ATDC5 Cells

• Published in: Current topics in nutraceuticals research Vol. 18; no. 1; pp. 108 - 114
• Main Authors: Liu, Haifeng, Zhang, Fan, Wu, Huiming, Wang, Qinglai, Zhang, Kuixian, Zeng, Xiaoshuai, Prasad, Chandan
• Format: Journal Article
• Language: English
• Published: New Century Health Publishers, LLC 01.02.2020
• Subjects: Analysis; Anti-inflammatory agents; Apoptosis; Asian medicine; Cytokines; Elderly; Inflammation; Interleukins; Mitogens; Monosaccharides; Necrosis; Osteoarthritis; Protein kinases; Traditional Chinese medicine; Tumor necrosis factor; Tumors; China
• ISSN: 1540-7535; 2641-452X
• DOI: 10.37290/ctnr2641-452x.18:108-114

Osteoarthritis is a bone-joint disease prevalent in older people characterized by joint inflammation. In traditional Chinese medicine, polydatin plays an important anti-inflammatory role. This study analyzed the potential effects and possible internal mechanisms of polydatin on osteoarthritis. First, lipopolysaccharide-induced osteoarthritis injury was established in chondrogenic ATDC5 cells. Lipopolysaccharides significantly stimulated inflammatory injuries in ATDC5 cells as exemplified by a decrease in cell viability and an increase in inflammatory cytokine secretions including interleukin-6, tumor necrosis factor-[alpha], and interleukin-1. Moreover, lipopolysacchrides also increased Cleaved caspase-3 and Cleaved Poly (ADP-ribose) polymerase to promote cell apoptosis. Second, polydatin showed significant protective effects against lipopolysaccharide-induced inflammatory injury, again exemplified by increased cell viability, decreased inflammatory cytokines, Cleaved caspase-3, and Cleaved poly (ADP-ribose) polymerase. Lastly, miR-125b and its binding target Rho-Associated Coiled-Coil Containing Protein Kinase 1 were closely associated with regulatory effects of polydatin against lipopolysaccharide-stimulated ATDC5 cell inflammatory injuries. Polydatin alleviated lipopolysaccharide-stimulated inflammatory injuries via the down-regulation of miR-125b. The present study concludes that polydatin plays an anti-inflammatory role in lipopolysaccharide-stimulated ATDC5 cell inflammatory injuries via the down-regulation of miR-125b. Keywords: Chondrogenic ATDC5; Inflammation; Lipopolysaccharide; miR-125b; Polydatin; ROCK1