Exploring the Mycovirus Sclerotinia sclerotiorum Hypovirulence-Associated DNA Virus 1 as a Biocontrol Agent of White Mold Caused by Sclerotinia sclerotiorum

is a necrotrophic fungal pathogen causing white mold on many important economic crops. Recently, some mycoviruses such as hypovirulence-associated DNA virus 1 (SsHADV-1) converted into a beneficial symbiont that helps plants manage pathogens and other stresses. To explore the potential use of SsHADV...

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Published inPlant disease Vol. 108; no. 3; p. 624
Main Authors Fu, Min, Qu, Zheng, Pierre-Pierre, Nickisha, Jiang, Daohong, Souza, Fernanda L, Miklas, Phillip N, Porter, Lyndon D, Vandemark, George J, Chen, Weidong
Format Journal Article
LanguageEnglish
Published United States 01.03.2024
Subjects
Ascomycota - genetics
DNA Viruses - genetics
Fungal Viruses
Satellite Viruses
SsHADV-1
Sclerotinia sclerotiorum
biopriming
gene expression
white mold
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Summary:is a necrotrophic fungal pathogen causing white mold on many important economic crops. Recently, some mycoviruses such as hypovirulence-associated DNA virus 1 (SsHADV-1) converted into a beneficial symbiont that helps plants manage pathogens and other stresses. To explore the potential use of SsHADV-1 as a biocontrol agent in the United States and to test the efficacy of SsHADV-1-infected United States isolates in managing white mold and other crop diseases, SsHADV-1 was transferred from the Chinese strain DT-8 to United States isolates of . SsHADV-1 is readily transmitted horizontally among United States isolates of and consistently conferred hypovirulence to its host strains. Biopriming of dry bean seeds with hypovirulent strains enhanced resistance to white mold, gray mold, and Rhizoctonia root rot. To investigate the underlying mechanisms, endophytic growth of hypovirulent in dry beans was confirmed using PCR, and the expression of 12 plant defense-related genes were monitored before and after infection. The results indicated that the endophytic growth of SsHADV-1-infected strains in plants stimulated the expression of plant immunity pathway genes that assisted a rapid response from the plant to fungal infection. Finally, application of the seed biopriming technology with SsHADV-1-infected hypervirulent strain has promise for the biological control of several diseases of wheat, pea, and sunflower.
ISSN:0191-2917
DOI:10.1094/PDIS-07-23-1458-RE