Chitinase 3-like 1 gene (T/C) polymorphism and serum YKL-40 levels in patients with hepatocellular carcinoma

• Published in: Meta Gene Vol. 24; p. 100686
• Main Authors: Saleh, Amany A., Alhanafy, Alshimaa M., Elbahr, Osama, El-Hefnawy, Sally M.
• Format: Journal Article
• Language: English
• Published: Elsevier B.V 01.06.2020
• Subjects: Chitinase 3-like 1; HCC; Polymorphism; YKL-40; HCC; YKL-40; Chitinase 3-like 1; Polymorphism
• ISSN: 2214-5400; 2214-5400
• DOI: 10.1016/j.mgene.2020.100686

The chitinase 3-like 1 (CHI3L1) gene encodes the YKL-40 protein. Serum YKL-40 levels are controlled by polymorphisms in the CHI3L1 gene. We aimed to examine the association of the rs880633 (T/C) polymorphism in the CHI3L1 gene with serum YKL-40 levels and to evaluate the role of this polymorphism as a risk factor for HCC and its effect on patient survival. A total of 225 subjects were categorized into two groups: group I included 120 patients with HCC, and group II involved 105 age and gender-matched healthy volunteers. Genotypes of the rs880633 (T/C) polymorphism in the CHI3L1 gene were analyzed using an allele discrimination assay with real-time PCR, and serum YKL-40 levels were determined using an ELISA technique. A significant difference in the genotype frequency of the T/C polymorphism in the CHI3L1 gene was observed between the two groups, with an increase in the frequency of the CC genotype observed among patients with HCC. Regarding the allelic distribution, the C allele was significantly predominant in patients, with HCC presenting with an increased HCC risk compared to the T allele. Subjects with the CC genotype had the highest serum YKL-40 levels, followed by those with the TC genotype, while subjects with the TT genotype had the lowest serum YKL-40 levels. An analysis of the overall survival of HCC patients revealed that patients with both the CC and CT genotypes displayed a significantly shorter survival rate than patients carrying the TT genotype. The rs880633 polymorphism in the CHI3L1 gene may be a risk element for HCC. Patients with the CC genotype displayed the highest serum YKL-40 levels, which were linked to poor prognosis. •SNP rs880633 at CHI3L1 gene may be a potential risk factor for HCC progression.•HCC patients carrying CC and TC genotypes have the highest serum CHI3L1 or YKL40 levels•HCC patients carrying CC genotype have bad prognosis and short survival•SNP rs880633 at CHI3L1 gene could predict the overall survival and disease outcome.