Induction of plasmacytomas that secrete monoclonal anti-peptide antibodies by retroviral transformation
ABL-MYC, a retrovirus that coexpresses v- abl and c- myc, was used to infect six BALB/c mice that had been immunized twice with a KLH-conjugated peptide that consisted of the 18 carboxyterminal amino acids of protein kinase C-η (PKC-η). All mice developed transplantable, monoclonal plastomas, and fi...
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Published in | Journal of immunological methods Vol. 168; no. 1; pp. 123 - 130 |
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Main Authors | , , , , |
Format | Journal Article |
Language | English |
Published |
Netherlands
Elsevier B.V
12.01.1994
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Subjects | |
Online Access | Get full text |
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Summary: | ABL-MYC, a retrovirus that coexpresses v-
abl and c-
myc, was used to infect six BALB/c mice that had been immunized twice with a KLH-conjugated peptide that consisted of the 18 carboxyterminal amino acids of protein kinase C-η (PKC-η). All mice developed transplantable, monoclonal plastomas, and five out of six plamacytomas secreted antigen-specific antibodies, even after transplantation. All these antibodies recognized PKC-η on Western blots of crude cell lysates and did not cross react with other isoforms of the PKC family. |
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Bibliography: | ObjectType-Article-2 SourceType-Scholarly Journals-1 ObjectType-Feature-1 content type line 23 ObjectType-Article-1 ObjectType-Feature-2 |
ISSN: | 0022-1759 1872-7905 |
DOI: | 10.1016/0022-1759(94)90216-X |