Induction of plasmacytomas that secrete monoclonal anti-peptide antibodies by retroviral transformation

• Published in: Journal of immunological methods Vol. 168; no. 1; pp. 123 - 130
• Main Authors: Weissinger, Eva M., Henderson, Darren W., Mischak, Harald, Goodnight, JoAnne, Frederic Mushinski, J.
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 12.01.1994
• Subjects: ABL-MYC; Amino Acid Sequence; Animals; Antibodies, Monoclonal - biosynthesis; Antibody Specificity; Cell Transformation, Neoplastic - genetics; Cell Transformation, Neoplastic - immunology; Cell Transformation, Viral - genetics; Cell Transformation, Viral - immunology; Hemocyanins - immunology; Isoenzymes - immunology; Mice; Mice, Inbred BALB C; Molecular Sequence Data; Monoclonal antibody; Neoplasms, Experimental - immunology; Oncogene Proteins v-abl - genetics; Plasmacytoma - immunology; Plasmacytoma - metabolism; Plasmacytoma - microbiology; Protein Kinase C - immunology; Protein kinase C-η; Proto-Oncogene Proteins c-myc - genetics; Retroviridae - genetics; Retrovirus; PBS; Protein kinase C-η; TBS; FCA; KLH; PKC; g0; i.p; g1; Monoclonal antibody; ABL-MYC; Retrovirus; DMEM; SDS-PAGE; ffu; FCS; FIA
• ISSN: 0022-1759; 1872-7905
• DOI: 10.1016/0022-1759(94)90216-X

ABL-MYC, a retrovirus that coexpresses v- abl and c- myc, was used to infect six BALB/c mice that had been immunized twice with a KLH-conjugated peptide that consisted of the 18 carboxyterminal amino acids of protein kinase C-η (PKC-η). All mice developed transplantable, monoclonal plastomas, and five out of six plamacytomas secreted antigen-specific antibodies, even after transplantation. All these antibodies recognized PKC-η on Western blots of crude cell lysates and did not cross react with other isoforms of the PKC family.