Transcutaneous Measurement of Glomerular Filtration Rate in Rodents

• Published in: Diabetic Nephropathy Vol. 2067; pp. 129 - 137
• Main Authors: Daniele, Cristina, Nardozi, Daniela, Torelli, Angelo, Khan, Arif ul Maula, Gretz, Norbert
• Format: Book Chapter Journal Article
• Language: English
• Published: New York, NY Springer US 2020
• Series: Methods in Molecular Biology
• Subjects: Animals; Fluorescent Dyes - administration & dosage; Fluorescent Dyes - analysis; Fluorescent Dyes - pharmacokinetics; GFR marker; Glomerular filtration rate; Glomerular Filtration Rate - physiology; Half-life calculation; Injections, Intravenous; Kidney - metabolism; Kidney Function Tests - instrumentation; Kidney Function Tests - methods; Mice; Rats; Renal Elimination - physiology; Renal function evaluation; Software; Spectroscopy, Near-Infrared - instrumentation; Spectroscopy, Near-Infrared - methods; Transcutaneous measurement; User-friendly software; User-friendly software; Transcutaneous measurement; Half-life calculation; GFR marker; Renal function evaluation; Glomerular filtration rate
• ISBN: 9781493998401; 1493998404
• ISSN: 1064-3745; 1940-6029
• DOI: 10.1007/978-1-4939-9841-8_9

Glomerular filtration rate (GFR) is considered the gold standard to test kidney function. However, the serial blood and/or urine sample collection required for the calculation of the GFR is stressful for the animal and time consuming for the experimenter. Here, we describe a transcutaneous assessment of renal function in conscious animals that does not require plasma or urine sampling and/or deep anesthesia. For the measurement, we use a near-infrared (NIR) device that records the excretion kinetic of the renal marker ABZWCY-HPβCD. ABZWCY-HPβCD is a new hydrophilic, stable, and nontoxic NIR fluorescent agent that can be used as a renal marker as it is filtrated and completely excreted through the kidneys into the urine without reabsorption or secretion and without accumulation in the skin. The data recorded in the device are then analyzed with “GFRmeasure,” an open-source, freely downloadable, and user-friendly software.