Simultaneous fixation of carbon dioxide and purification of undiluted swine slurry by culturing Chlorella vulgaris MBFJNU-1

• Published in: Algal research (Amsterdam) Vol. 47; p. 101866
• Main Authors: Zheng, Mingmin, Ji, Xiaowei, He, Yongjin, Li, Zhefu, Wang, Mingzi, Chen, Bilian, Huang, Jian
• Format: Journal Article
• Language: English
• Published: Elsevier B.V 01.05.2020
• Subjects: Carbon dioxide; Chlorella vulgaris; Proteomic analysis; Swine slurry; Swine slurry; Proteomic analysis; Chlorella vulgaris; Carbon dioxide
• ISSN: 2211-9264; 2211-9264
• DOI: 10.1016/j.algal.2020.101866

Microalgae biotechnology for wastewater treatment is a promising strategy to solve some severe environmental problems. In this work, carbon dioxide (CO2) fixation rate and performance of wastewater treatment by a unique Chlorella vulgaris MBFJNU-1 were deeply evaluated using undiluted swine slurry. The obtained results showed that C. vulgaris MBFJNU-1 achieved the highest biomass (3.08 g/L) under the optimized conditions (5% CO2, 24 h/d ventilation time and 1000 mL/min ventilation rate). Meantime, the biomass had 54.7% of protein and 36.25 mg/g of chlorophyll. Moreover, in the end of trials, C. vulgaris MBFJNU-1 to treat swine slurry attained the total nitrogen removal rate of 74%, ammonia nitrogen removal rate of 78% and total phosphorus removal rate of 87%. The greatest CO2 fixation rate (454 mg/(L·d)) was obtained by the microalgae. To illustrate the CO2 fixation and nutrients removal rates of wastewater, proteomic analysis for C. vulgaris MBFJNU-1 was assayed and results showed that a certain amount of CO2 could promote some enzymes in photosynthesis and nitrogen metabolism, which was consistent with the experimental results. Taken together, C. vulgaris MBFJNU-1 was the potential microalgae to effectively fix CO2 and remove some nutrients from undiluted swine slurry for microalgae biomass. [Display omitted] •Fixation of CO2 by Chlorella vulgaris MBFJNU-1 with a carbon fixation rate of 454 mg/(L·d)•Purification of undiluted swine slurry by culturing Chlorella vulgaris MBFJNU-1•Proteomic analysis revealed CO2 could promote photosynthesis and up-regulate enzymes in corresponding metabolic pathways.•CO2 affect nitrogen metabolism pathways of Chlorella vulgaris MBFJNU-1.