Live Imaging of Arabidopsis Axillary Meristems

Axillary meristems (AMs) are established postembryonically at the leaf axils and can develop into lateral branches. The initiation of AMs establishes new growth axis and is of primary importance for understanding plant development. Understanding plant development requires live imaging of morphogenes...

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Bibliographic Details
Published inMethods in molecular biology (Clifton, N.J.) Vol. 2094; p. 59
Main Authors Shi, Bihai, Wang, Hongli, Jiao, Yuling
Format Journal Article
LanguageEnglish
Published United States 01.01.2020
Subjects
Arabidopsis - metabolism
Cell Culture Techniques - methods
Cells, Cultured
Meristem - metabolism
Microscopy, Confocal - instrumentation
Microscopy, Confocal - methods
Plant Leaves - metabolism
Confocal
In vitro culture
Live imaging
Axillary meristem
Arabidopsis
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Summary:Axillary meristems (AMs) are established postembryonically at the leaf axils and can develop into lateral branches. The initiation of AMs establishes new growth axis and is of primary importance for understanding plant development. Understanding plant development requires live imaging of morphogenesis and gene expression. However, AMs are embedded in the leaf axil, making it challenging to perform live imaging. In this chapter, we describe how to prepare and culture Arabidopsis thaliana leaves in vitro, to perform one-time or time-lapse imaging of AM initiation with a confocal microscope.
ISSN:1940-6029
DOI:10.1007/978-1-0716-0183-9_7