Sleep deprivation boosts O2·− levels in the brains of mice as visualized by a Golgi apparatus–targeted ratiometric fluorescence nanosensor

• Published in: Mikrochimica acta (1966) Vol. 191; no. 5; p. 265
• Main Authors: Song, Wei, Yao, Chunxia, Lu, Yangyang, Qian, Qunli, Wu, Jun, Shi, Wenru, Li, Huiru, Huang, Hong, Wang, Weikang, Song, Weiguo
• Format: Journal Article
• Language: English
• Published: Vienna Springer Vienna 01.05.2024; Springer Nature B.V
• Subjects: Analytical Chemistry; Brain; Characterization and Evaluation of Materials; Chemistry; Chemistry and Materials Science; Emission; Fluorescence; Microengineering; Nanochemistry; Nanosensors; Nanotechnology; Original Paper; Reference signals; Sleep deprivation; Mouse brain; Carbon nanodots; Polymer dots; Sleep deprivation; Golgi apparatus
• ISSN: 0026-3672; 1436-5073
• DOI: 10.1007/s00604-024-06352-x

Sleep deprivation (SD) is highly prevalent in the modern technological world. Emerging evidence shows that sleep deprivation is associated with oxidative stress. At the organelle level, the Golgi apparatus actively participates in the stress response. In this study, to determine whether SD and Golgi apparatus stress are correlated, we rationally designed and fabricated a novel Golgi apparatus–targeted ratiometric nanoprobe called Golgi dots for O 2 · − detection. This probe exhibits high sensitivity and selectivity in cells and brain slices of sleep-deprived mice. Golgi dots can be readily synthesized by coprecipitation of Golgi-F127, an amphiphilic polymer F127 modified with a Golgi apparatus targeting moiety, caffeic acid (CA), the responsive unit for O 2 · − , and red emissive carbon nanodots (CDs), which act as the reference signal. The fluorescence emission spectrum of the developed nanoprobe showed an intense peak at 674 nm, accompanied by a shoulder peak at 485 nm. As O 2 · − was gradually added, the fluorescence at 485 nm continuously increased; in contrast, the emission intensity at 674 nm assigned to the CDs remained constant, resulting in the ratiometric sensing of O 2 · − . The present ratiometric nanoprobe showed high selectivity for O 2 · − monitoring due to the specific recognition of O 2 · − by CA. Moreover, the Golgi dots exhibited good linearity with respect to the O 2 · − concentration within 5 to 40 μM, and the limit of detection (LOD) was ~ 0.13 μM. Additionally, the Golgi dots showed low cytotoxicity and an ability to target the Golgi apparatus. Inspired by these excellent properties, we then applied the Golgi dots to successfully monitor exogenous and endogenous O 2 · − levels within the Golgi apparatus. Importantly, with the help of Golgi dots, we determined that SD substantially elevated O 2 · − levels in the brain. Graphical Abstract