Densitometric determination of human papillomavirus DNA quantities by chromato-scanning in the fluorescence mode

The quantitation of human papillomavirus DNA isolated from warts by chromato-scanning (fluorescence mode) photographs of ethidium bromide-stained agarose gels is described. Excitation at 200 nm (with a cutoff filter at 400 nm) generates fluorescence from the white portion of the printing paper. The...

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Published inJournal of virological methods Vol. 32; no. 1; pp. 101 - 108
Main Authors Masanobu, Chinami, Kentaro, Yuge, Hiroshi, Hachisuka, Eiko, Tanikawa, Masashi, Goto, Yasushi, Ohtsu, Yoichiro, Sasai, Masahisa, Shingu
Format Journal Article
LanguageEnglish
Published Netherlands Elsevier B.V 01.04.1991
Subjects
Bacteriophage lambda - genetics
Chromato-scanning
Densitometry
DNA, Viral - analysis
DNA, Viral - isolation & purification
Electrophoresis, Agar Gel
Fluorescence
Human papillomavirus
Humans
Papillomaviridae - genetics
Photography
Warts - microbiology
Chromato-scanning
Human papillomavirus
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Summary:The quantitation of human papillomavirus DNA isolated from warts by chromato-scanning (fluorescence mode) photographs of ethidium bromide-stained agarose gels is described. Excitation at 200 nm (with a cutoff filter at 400 nm) generates fluorescence from the white portion of the printing paper. The fluorescent intensity correlated with the quantities of DNA in the band of interest. The amounts of DNA were determined using calibration curves of approximately the same size as lambda phage DNA fragments. This general method of quantification is applicable to photographs of other types of polynucleotides capable of being separated and stained in a gel medium.
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ISSN:0166-0934
1879-0984
DOI:10.1016/0166-0934(91)90189-7