Sumoylation of PDX-1 Regulates the Rat Insulin 2 Gene Transcription

• Published in: Applied Mechanics and Materials Vol. 556-562; pp. 253 - 256
• Main Authors: Jian, Ruo Lei, Wang, Feng Po, Wang, Nan, Mao, Li Bin, Zhang, Tong Cun
• Format: Journal Article
• Language: English
• Published: Zurich Trans Tech Publications Ltd 01.05.2014
• Subjects: Sumoylation; SUMO-1; Insulin; PDX-1
• ISBN: 3038351156; 9783038351153
• ISSN: 1660-9336; 1662-7482; 1662-7482
• DOI: 10.4028/www.scientific.net/AMM.556-562.253

Insulin is essential for maintaining glucose homeostasis and is synthesized and secreted almost exclusively from pancreatic β-cells in the islets of Langerhans. The analysis of rat insulin2 promoter sequences indicates that it has pancreatic and duodenal homeobox factor-1 (PDX-1) binding site. In mature β-cells, PDX-1 transactivates the insulin and other genes involved in glucose sensing and metabolism. The function of PDX-1 can be regulated by post-translational modifications. Sumoylation plays an important role in regulating numerous physiological and pathological processes through altering the function of its target proteins. In this paper, to understand the function and transcription activities of insulin gene promoter, we constructed rat insulin 2 promoter (RIP II). Then we investigated whether sumoylation of PDX-1 activated this promoter transcription by luciferase reporter assay in HepG-2 cells. Results indicated that sumoylation enhanced PDX-1 activity for the insulin gene promoter transcription.