Use of antioxidants to extend the storage of lyophilized cell-free synthesis system

Modern chemical processes, vital for diverse product manufacturing, often result in substantial energy consumption and environmental waste. As environmental concerns continue to escalate and industries evolve to meet customized demands, biomanufacturing emerges as a promising alternative due to its...

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Published inBiotechnology and bioprocess engineering Vol. 29; no. 2; pp. 263 - 269
Main Authors Kim, Kyu Jae, Lee, So Jeong, Kim, Dong-Myung
Format Journal Article
LanguageEnglish
Published Seoul The Korean Society for Biotechnology and Bioengineering 01.04.2024
Springer Nature B.V
Subjects
Antioxidants
Biomolecules
biosynthesis
Biotechnology
Chemical reactions
Chemical synthesis
Chemistry
Chemistry and Materials Science
Dithiothreitol
energy
Energy consumption
freeze drying
Industrial and Production Engineering
Production methods
protein synthesis
Research Paper
shelf life
Sodium nitrite
wastes
Antioxidants
Lyophilization
On-demand biomanufacturing
Cell-free synthesis
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Summary:Modern chemical processes, vital for diverse product manufacturing, often result in substantial energy consumption and environmental waste. As environmental concerns continue to escalate and industries evolve to meet customized demands, biomanufacturing emerges as a promising alternative due to its efficiency, expandability, and eco-friendliness. Cell-free synthesis systems, which harness cellular extracts for biosynthetic reactions, offer a highly adaptable solution for biomanufacturing, particularly when rapid production is required with limited resources. While conventional cell-free systems encounter challenges related to storage and transportation due to the necessity for ultra-cold temperatures, recent studies have demonstrated that these systems can be lyophilized and rehydrated to enable on-demand biomolecule production. Our study aims to enhance the stability of lyophilized cell-free systems. We have discovered the significant role played by antioxidants, specifically dithiothreitol and sodium nitrite, in preserving translational activity during extended storage. This finding represents a significant step forward for decentralized, on-demand protein production using cell-free methods.
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ISSN:1226-8372
1976-3816
DOI:10.1007/s12257-024-00054-0