The sigma-70 subunit from Escherichia coli C differs from that of E. coli K-12

• Published in: Gene Vol. 162; no. 1; pp. 161 - 162
• Main Authors: Christie, Gail E., Cale, Sara B.
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 30.08.1995
• Subjects: Amino Acid Sequence; Bacterial Proteins - genetics; Base Sequence; DNA-Directed RNA Polymerases - genetics; Escherichia coli; Escherichia coli - classification; Escherichia coli - enzymology; Escherichia coli - genetics; Genes, Bacterial; Molecular Sequence Data; Nucleotide sequence; Point Mutation; RNA polymerase; Sequence Deletion; Sigma Factor - genetics; Species Specificity; σ factor; aa, amino acid(s); Nucleotide sequence; nt, nucleotide(s); CAP, catabolite gene activator protein; rpoD, gene encoding the primary sigma-70 ( σ 70) subunit of RNA polymerase; bp, base pair(s); σ factor; RNA polymerase
• ISSN: 0378-1119; 1879-0038
• DOI: 10.1016/0378-1119(95)00333-2

The nucleotide sequence of the rpoD gene (encoding the primary sigma-70 ( σ 70) subunit of RNA polymerase) from Escherichia coli C was determined. This gene differs from that of E. coli K-12 by a 30-bp deletion and five single-bp substitutions, resulting in a σ 70 subunit with three amino acid (aa) changes and a deletion of ten acidic aa.