Cloned primed lymphocyte test cells recognize the fourth, fifth, and sixth hypervariable regions at amino acid positions 65–87 of the DPB1 molecule

• Published in: Human immunology Vol. 42; no. 2; pp. 123 - 130
• Main Authors: Naruse, Taeko K., Nose, Yoshisuke, Kagiya, Masahiko, Liao, Geng, Nabeya, Noboru, Kimura, Minoru, Isshiki, Gen, Inoko, Hidetoshi
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 01.02.1995
• Subjects: Amino Acid Sequence; Base Sequence; Genotype; HLA-DP Antigens - genetics; HLA-DP Antigens - immunology; HLA-DP beta-Chains; Humans; Lymphocyte Activation - immunology; Molecular Sequence Data; Polymerase Chain Reaction; Polymorphism, Genetic - genetics; Polymorphism, Genetic - immunology; T-Lymphocytes - immunology
• ISSN: 0198-8859; 1879-1166
• DOI: 10.1016/0198-8859(94)00084-4

Genetic polymorphisms of the HLA-DPB 1 gene in Japanese and Caucasian panel cells defined by PLT were analyzed by the PCR-based genotyping technique PCR-RFLP, and suballeles of DPw3 (DPB 1 ∗03) and DP“Cp63” (DPB 1 ∗09) could be detected. PLT-defined DPw3 cells were typed by PCR-RFLP as either DPB 1 ∗0301 or DPB 1 ∗1401. On the other hand, PLT-defined DPCp63-typed cells were typed as DPB 1 ∗0901 or DPB 1 ∗1001. These results indicate that both DPw3 and DPCp63 are split into two subantigens. DPw2 and DPw4 are DPB 1 ∗0201 and 0202 and DPB 1 ∗0401 and 0402, respectively. Comparative analysis of the amino acid sequences of the DPw2-, DPw4-, DPw3-, and DPCp63-associated alleles revealed that the fourth (C), fifth (D), and sixth (E) hypervariable regions at amino acid positions 65–87 were shared within the same PLT-defined DP antigen groups, suggesting that these three hypervariable regions are recognized by cloned T cells in PLT, thus determining DP antigen specificity. On the basis of this model, 44 DPB 1 alleles can be classified into 18 antigen groups, each of which may possibly represent a PLT-defined single DP specificity.