Antibiotic Resistance Characterization of Bacteria Isolated from Traditional Chinese Paocai

• Published in: Current microbiology Vol. 78; no. 11; pp. 3853 - 3862
• Main Authors: Li, Mei, Tang, Yao, Guo, Liyan, Lei, Tao, Deng, Yunfei, Wang, Liang, Zhang, Qisheng, Li, Chengkang
• Format: Journal Article
• Language: English
• Published: New York Springer US 01.11.2021; Springer Nature B.V
• Subjects: Ampicillin; Antibiotic resistance; Antibiotics; Bacteria; Biomedical and Life Sciences; Biotechnology; Ciprofloxacin; Citrobacter; Drug resistance; Erythromycin; Genes; Klebsiella; Life Sciences; Microbiology; Minimum inhibitory concentration; Sodium; Strains (organisms); Streptomycin; Streptomycin sulfate; Sulfate resistance; Sulfates; TetM gene
• ISSN: 0343-8651; 1432-0991
• DOI: 10.1007/s00284-021-02629-z

In this work, the antibiotic resistance of 218 isolates to 9 different antibiotics was analyzed with minimum inhibitory concentration method. All Lactobacillus pentosus strains were found to be resistant to streptomycin sulfate and ciprofloxacin hydrochloride. Lactococcus lactis strains were resistant to streptomycin sulfate. Specifically, 90% Klebsiella oxytoca and all Citrobacter freundii strains were resistant to ampicillin sodium. 30% K. oxytoca strains were resistant to ciprofloxacin hydrochloride. All Bacillus albus strains were resistant to erythromycin and 80% strains were resistant to ampicillin sodium. Results from PCR analysis revealed that 90 isolates carried the aad E gene. The tet M gene was detected in four L. pentosus isolates. And the streptomycin resistant gene aad A was detected in one L. pentosus isolate. Metagenome analysis revealed that 74.7% genes associated with antibiotic resistance were antibiotic resistance genes. The tet M and aad A genes, detected in PCR analysis, were also retrieved from the paocai metagenome. In brief, this study generated the antibiotic resistance profile of some paocai-originated bacteria strains. L. pentosus found in the final edible paocai were inherently resistant to antibiotics, such as streptomycin and ciprofloxacin. Results in this work reminds us to carefully choose the LAB strains for traditional Chinese paocai production to avoid potential spreading of antibiotic resistant genes.