Detection of influenza A virus using carbon nanotubes field effect transistor based DNA sensor

• Published in: Physica. E, Low-dimensional systems & nanostructures Vol. 93; pp. 83 - 86
• Main Authors: Tran, Thi Luyen, Nguyen, Thi Thuy, Huyen Tran, Thi Thu, Chu, Van Tuan, Thinh Tran, Quang, Tuan Mai, Anh
• Format: Journal Article
• Language: English
• Published: Elsevier B.V 01.09.2017
• Subjects: Carbon nanotubes; DNA sensor; Field effect transistor; Influenza A virus; Field effect transistor; Carbon nanotubes; Influenza A virus; DNA sensor
• ISSN: 1386-9477; 1873-1759
• DOI: 10.1016/j.physe.2017.05.019

The carbon nanotubes field effect transistor (CNTFET) based DNA sensor was developed, in this paper, for detection of influenza A virus DNA. Number of factors that influence the output signal and analytical results were investigated. The initial probe DNA, decides the available DNA strands on CNTs, was 10µM. The hybridization time for defined single helix was 120min. The hybridization temperature was set at 30°C to get a net change in drain current of the DNA sensor without altering properties of any biological compounds. The response time of the DNA sensor was less than one minute with a high reproducibility. In addition, the DNA sensor has a wide linear detection range from 1pM to 10nM, and a very low detection limit of 1pM. Finally, after 7-month storage in 7.4pH buffer, the output signal of DNA sensor recovered 97%. •The CNTFET based DNA sensor was for detection of influenza type A virus DNA.•Factors that influence the output signal of the DNA sensor were investigated.•The sensor has detection limit of 1pM in the linear range of 1pM to 10nM.•The response time of DNA sensor is less than one minute.•After 7-months storage the ID of the DNA sensor recovered 97%.