Augmentative effect of OK-432 and/or Nocardia rubra cell wall skeleton on superoxide generation from polymorphonuclear leukocytes

• Published in: Japanese Journal of Clinical Immunology Vol. 12; no. 6; pp. 608 - 614
• Main Authors: Yoshikawa, Toshikazu, Takano, Hirohisa, Naito, Yuji, Ichikawa, Hiroshi, Takahashi, Shuji, Tasaki, Naohiro, Yasuda, Mitsunori, Sugino, Shigeru, Kondo, Motoharu
• Format: Journal Article
• Language: English
• Published: The Japan Society for Clinical Immunology 1989
• Subjects: Cypridana luciferin analog-dependent chemiluminescence; Nocardia rubra cell wall skeleton; OK-432; Polymorphonuclear leukocytes; Superoxide generation
• ISSN: 0911-4300; 1349-7413
• DOI: 10.2177/jsci.12.608

Effects of OK-432 and/or Nocardia rubra cell wall skeleton (NCWS) on superoxide generation from polymorphonuclear leukocytes (PMN) were studied by the new method of Cypridana luciferin analog-dependent chemiluminescence. Superoxide generation from the rat peritoneal PMN stimulated by phorbol myristate acetate or opsonized zymosan was augmented by the 8-day subcutaneous injection of OK-432 or NCWS in vivo, but neither by the 3-day subcutaneous injection nor by the incubation of PMN with OK-432 or NCWS in vitro. Combined 8-day subcutaneous treatment with OK-432 and NCWS induced marked increase in the superoxide generation from PMN stimulated by opsonized zymosan. OK-432 or NCWS did not directly stimulate PMN to generate superoxide under the condition we tested. It is suggested that the increased generation of oxygen radicals induced by OK-432 and/or NCWS may play a beneficial role in the tumoricidal activity exhibited by PMN, and that the augmentative offect of OK-432 and/or NCWS may not be direct.