Genetic characteristics and growth patterns of the hybrid grouper derived from the hybridization of Epinephelus fuscoguttatus (female) × Epinephelus polyphekadion (male)

• Published in: Journal of fish biology Vol. 102; no. 2; pp. 328 - 339
• Main Authors: Cao, Liu, Chen, Pan, Hou, Xingrong, Ma, Jun, Yang, Ning, Xu, Yanping, Zhang, Yanjin, Zhao, Anqi, Zhang, Jiyuan, Li, Xinyu, Huang, Hai
• Format: Journal Article
• Language: English
• Published: Oxford, UK Blackwell Publishing Ltd 01.02.2023; Wiley Subscription Services, Inc
• Subjects: Allometry; Animals; Base Sequence; Bass; Body height; Body mass; Chromosomes; Epinephelus fuscoguttatus; Evolution; Evolution & development; Female; Females; Flow cytometry; genetic characteristics; Genetic diversity; Genetic variation; growth pattern; Growth patterns; Height; hybrid grouper; Hybridization; Hybridization, Genetic; Male; Males; Marine fishes; Mass; Nucleotides; Ploidy; rDNA family; rDNA family; genetic characteristics; growth pattern; hybrid grouper
• ISSN: 0022-1112; 1095-8649
• DOI: 10.1111/jfb.15263

Hybridization is one of the primary methods used to cultivate farmed grouper species. The hybrid grouper derived from crossing Epinephelus fuscoguttatus (♀) and E. polyphekadion (♂) exhibits growth superiority over its parents. The genetic characteristics and growth patterns of the hybrid grouper have not yet been defined. This study confirms the ploidy level of the hybrid grouper (2n = 48) using chromosome count analysis and flow cytometry. The 5S rDNA family was used to evaluate genetic diversity. Only one 5S class (~400 bp) was detected in the hybrid grouper, which could be used to distinguish between two different types based on nucleotide sequences, likely representing homologous unit classes from the female and male parental species. Growth patterns of 5–8‐month‐old hybrid groupers were also monitored. In this phase, a positive allometric growth pattern in body mass with total length was found. Body height and body mass were significantly correlated based on correlation and path coefficient, suggesting that body height could serve as an excellent index to increase body mass. These results aid our understanding of the genetic evolution of the hybrid grouper and inform the development of improved rearing techniques.