Immunolocalization of glucose transporter GLUT4 within human skeletal muscle

To investigate the cellular and subcellular distribution of glucose transporters in skeletal muscle, the glucose transporter isoform GLUT4 was localized in human muscle by electron microscopy via immunogold labeling with monoclonal (1F8) or COOH-terminal peptide polyclonal (ECU4) antibody and in iso...

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Published inDiabetes (New York, N.Y.) Vol. 40; no. 1; pp. 150 - 154
Main Authors FRIEDMAN, J. E, DUDEK, R. W, WHITEHEAD, D. S, DOWNES, D. L, FRISELL, W. R, CARO, J. F, DOHM, G. L
Format Journal Article
LanguageEnglish
Published Alexandria, VA American Diabetes Association 1991
Subjects
Animals
Biological and medical sciences
Biopsy
Cell Membrane - chemistry
Cell Membrane - ultrastructure
Fundamental and applied biological sciences. Psychology
Humans
Male
Microscopy, Immunoelectron
Monosaccharide Transport Proteins - analysis
Muscles - chemistry
Muscles - cytology
Muscles - ultrastructure
Rats
Rats, Inbred Strains
Reference Values
Sarcolemma - chemistry
Sarcolemma - ultrastructure
Striated muscle. Tendons
Vertebrates: osteoarticular system, musculoskeletal system
Human
Immunocytochemistry
Biological transport
Immunoblotting assay
Glucose
Striated muscle
Carrier protein
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Summary:To investigate the cellular and subcellular distribution of glucose transporters in skeletal muscle, the glucose transporter isoform GLUT4 was localized in human muscle by electron microscopy via immunogold labeling with monoclonal (1F8) or COOH-terminal peptide polyclonal (ECU4) antibody and in isolated rat membranes by Western blot. There was no labeling of GLUT4 in endothelial cells of the capillaries. There also was no labeling of GLUT4 on the surface plasma membrane (sarcolemma) under either basal or insulin-stimulated conditions. Specific labeling for GLUT4 was clearly observed in two compartments: within the triad (on terminal cisternae and transverse tubules) and on an intracellular compartment, possibly sarcoplasmic tubules. Isolated triad membranes from rat muscle also contained substantial quantities of GLUT4 transporter, but there was no detectable GLUT4 protein in isolated sarcolemmal membranes. These data suggest a possible mechanism that involves glucose transport across the muscle cell at the transverse tubule membrane, not the sarcolemma.
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ISSN:0012-1797
1939-327X
DOI:10.2337/diab.40.1.150