Primary structure and assignment to chromosome 6 of three related rat genes encoding liver serine protease inhibitors
Three closely related SPI genes which encode highly homologous proteins of the serine protease inhibitors family secreted by rat liver (SPI-1, SPI-2 and SPI-3), were isolated from genomic libraries and sequenced, totally ( SPI-2) or partially ( SPI-1 and SPI-3). These genes all map on rat chromosome...
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Published in | Gene Vol. 94; no. 2; pp. 273 - 282 |
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Main Authors | , , , , , , , , |
Format | Journal Article |
Language | English |
Published |
Lausanne
Elsevier B.V
1990
Amsterdam Elsevier New York, NY |
Subjects | |
Online Access | Get full text |
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Summary: | Three closely related
SPI genes which encode highly homologous proteins of the serine protease inhibitors family secreted by rat liver (SPI-1, SPI-2 and SPI-3), were isolated from genomic libraries and sequenced, totally (
SPI-2) or partially (
SPI-1 and
SPI-3). These genes all map on rat chromosome 6. Each of them spans about 10 kb and contains five exons separated by four introns, located at equivalent positions. S1 mapping analysis indicated that initiation of transcription occurs at the same position (
tsp) in each of the three genes. In vitro transcription experiments demonstrated the presence of promoter elements upstream from the putative
tsp. Detailed analysis of 5′-flanking sequences in the three
SPI revealed major differences. A high degree of identity (70%) was found within a 350-bp region preceding the ‘cap’ site, with the exception of a 42-bp spacer, which was only found in
SPI-3. Upstream from that point,
SPI-1 and
SPI-2 sequences remain largely homologous over at least 1 kb but completely diverge from the corresponding sequence in
SPI-3. This may, at least partly, account for the differential regulation of the three
SPI observed during acute inflammation and upon hypophysectomy. |
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Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 0378-1119 1879-0038 |
DOI: | 10.1016/0378-1119(90)90398-B |