Assignment of the porcine nuclear factor I/CTF (NFI/CTF) gene to chromosome 2q12-13 by FISH

• Published in: Mammalian genome Vol. 8; no. 6; p. 456
• Main Authors: Frönicke, L, Scherthan, H
• Format: Journal Article
• Language: English
• Published: United States Springer Nature B.V 01.06.1997
• Subjects: Animals; CCAAT-Enhancer-Binding Proteins; Chromosome 2; Chromosome Mapping; DNA-Binding Proteins - genetics; Humans; In Situ Hybridization, Fluorescence; NFI Transcription Factors; Nuclear factor I; Nuclear Proteins; Sequence Homology, Nucleic Acid; Swine - genetics; Transcription Factors; Y-Box-Binding Protein 1
• ISSN: 0938-8990; 1432-1777
• DOI: 10.1007/s003359900628

Species: Pig (Sus scrofa domestica). Locus name: Nuclear Factor I /CTF. Locus symbol: NFI/CTF. Map position: 2q12-13. Method of mapping: Fluorescence in situ hybridization (FISH). Molecular reagents: An EMBL3A phage containing 40 kb of pig NFI/CTF gene was biotinylated and detected by avidin-FITC. Previously identified homologs: NFIC maps to human Chromosome (Chr) 19p13. Discussion: Nuclear factor NFI/CTF is a member of a family of dimeric DNA-binding proteins. Its recognition sequence [TGG(C/A)N sub(5)] is present in a number of DNA viruses and in mammalian and avian genomes. The proteins are involved in cellular transcription regulation and adenovirus transcription initiation. In the human genome, NFI-A,B,C and NFI-X genes have been identified and mapped. NFI-C and NFI-X are located in close proximity on human Chr 19p13, whereas NFI-A and -B genes map to 1p31.2-31.3 and 9p24.1, respectively. In the mouse, Nfix has been assigned to 8C1-2. Of all mammalian nuclear factor I genes isolated to date, the genomic structure of the porcine gene has been characterized in most detail. Gross regional homologies between human and porcine genomes have recently been demarcated by Zoo-FISH. These studies revealed the presence of two conserved syntenic segments between HSA19 and SSC2cen-q21 and SSC6cen-q21. Comparative gene mapping data suggest that the p and q arms of HSA 19 are each conserved as a single block in artiodactyl genomes. The mapping of the porcine NFI/CTF gene to SSC2q12-13 corroborates the conserved synteny between HSA19p and the homologous segment at SSC2cen-q21, which has also been demonstrated by a recent forward and reverse Zoo-FISH approach.