Differential expression of phosphorylated Ca2+ /calmodulin-dependent protein kinase II and phosphorylated extracellular signal-regulated protein in the mouse hippocampus induced by various nociceptive stimuli

• Published in: Neuroscience Vol. 156; no. 3; pp. 436 - 449
• Main Authors: Seo, Y.-J, Kwon, M.-S, Choi, H.-W, Choi, S.-M, Kim, Y.-W, Lee, J.-K, Park, S.-H, Jung, J.-S, Suh, H.-W
• Format: Journal Article
• Language: English
• Published: United States 15.10.2008
• Subjects: Acetic Acid; Analysis of Variance; Animals; Behavior, Animal; Benzylamines - pharmacology; Calcium-Calmodulin-Dependent Protein Kinase Type 2 - metabolism; Extracellular Signal-Regulated MAP Kinases - metabolism; Flavonoids - pharmacology; Formaldehyde; Gene Expression Regulation, Enzymologic - drug effects; Glutamic Acid; Hippocampus - drug effects; Hippocampus - enzymology; Interleukin-1beta; Male; Mice; Mice, Inbred ICR; Neurology; Pain - chemically induced; Pain - metabolism; Pain Measurement - methods; Phosphorylation; Protein Kinase Inhibitors - pharmacology; Sulfonamides - pharmacology; Time Factors; Tumor Necrosis Factor-alpha; intrathecal; pain; hippocampus; phosphor-Ca 2+/calmodulin dependent protein kinase II alpha; pCaMK-IIα; phosphor-extracellular signal-regulated protein kinase; TBST; phosphate buffer; IL-1β; i.t; interleukin-1β; phospho-CaMKII; PB; TNF-α; CaMK-II; tumor necrosis factor-α; phospho-ERK; Ca 2+/calmodulin dependent protein kinase II; extracellular signal-regulated protein kinase; pERK; Tris-buffered saline containing 0.3% Tween-20; ERK
• ISSN: 0306-4522; 1873-7544
• DOI: 10.1016/j.neuroscience.2008.08.002

Abstract In the present study, we characterized differential expressions of phosphorylated Ca2+ /calmodulin-dependent protein kinase IIα (pCaMKIIα) and phosphorylated extracellular signal-regulated protein (pERK) in the mouse hippocampus induced by various nociceptive stimuli. In an immunoblot study, s.c. injection of formalin and intrathecal (i.t.) injections of glutamate, tumor necrosis factor-α (TNF-α), and interleukin-1β (IL-1 β) significantly increased pCaMKIIα expression in the hippocampus, but i.p. injections of acetic acid did not. pERK1/2 expression was also increased by i.t. injection of glutamate, TNF-α, and IL-1β but not by s.c. injections of formalin or i.p. injections of acetic acid. In an immunohistochemical study, we found that increased pCaMKIIα and pERK expressions were mainly located at CA3 or the dentate gyrus of the hippocampus. In a behavioral study, we assessed the effects of PD98059 (a MEK 1/2 inhibitor) and KN-93 (a CaMKII inhibitor) following i.c.v. administration on the nociceptive behaviors induced by i.t. injections of glutamate, pro-inflammatory cytokines (TNF-α or IL-1β), and i.p. injections of acetic acid. PD98059 as well as KN-93 significantly attenuated the nociceptive behavior induced by glutamate, pro-inflammatory cytokines, and acetic acid. Our results suggest that (1) pERKα and pCaMK-II located in the hippocampus are important regulators during the nociceptive processes induced by s.c. formalin, i.t. glutamate, i.t. pro-inflammatory cytokines, and i.p. acetic acid injection, respectively, and (2) the alteration of pERK and pCaMKIIα in nociceptive processing induced by formalin, glutamate, pro-inflammatory cytokines and acetic acid was modulated in a different manner.