Dual Therapy with Ellagic Acid and Carnosic Acid Ameliorates STZ-Induced Diabetic Conditions in Rats by Alleviating Liver Oxidative Stress

• Published in: Gazi tıp dergisi Vol. 36; no. 2; pp. 152 - 161
• Main Authors: Berktaş, Ayşegül, Gürsoy, Elif Naz, Kaltalıoğlu, Kaan, Coşkun Cevher, Şule
• Format: Journal Article
• Language: English
• Published: Galenos Publishing House 15.04.2025
• Subjects: carnosic acid; ellagic acid; inflammation; liver; oxidative stress
• ISSN: 1300-056X; 2147-2092
• DOI: 10.12996/gmj.2024.4162

Objective: Diabetes, a significant public health issue today, causes serious health problems due to its complications and will become a global epidemic if no action is taken. Diabetic patients experience elevated oxidative stress, which impairs wound healing. The purpose of this study was to investigate the effects of ellagic acid (EA) and carnosic acid (CA) on oxidative events in liver tissue in diabetic rats with wounds. Methods: The rats were divided into 7 groups as control, untreated-3 day, untreated-7 day, topical-3 day, topical-7 day, oral-3 day and oral-7 day. To induce diabetes in the subjects, each group received a single intraperitoneal injection of streptozotocin. Rats were treated topically or orally with EA + CA. On the 3rd and 7th days of recovery, the rats were sacrificed and the levels of nitric oxide (NOx), malondialdehyde (MDA), glutathione (GSH), protein carbonyls (PC), and ascorbic acid (AA) were measured spectrophotometrically to investigate the effects of oxidative stress in liver tissue. Results: Liver tissue MDA, NOx, and PC levels were determined to be statistically decreased in both topical and oral applications compared to the control and untreated groups (p<0.05). Liver tissue GSH, AA, and collagen levels were found to be statistically increased in both topical and oral applications when compared to the control and untreated groups (p<0.05). Conclusion: These results show that the combination of EA and CA with two different application methods significantly reduces oxidative stress associated with drug metabolism by affecting oxidative events in liver tissue, and has the potential to prevent possible complications of diabetes.