Diagnostic value of miRNA-101 and miRNA-27 in acute heart transplant rejection

• Published in: Vestnik transplantologii i iskusstvennykh organov Vol. 22; no. 4; pp. 20 - 26
• Main Authors: Velikiy, D. A., Gichkun, O. E., Sharapchenko, S. O., Mozheiko, N. P., Kurabekova, R. M., Shevchenko, O. P.
• Format: Journal Article
• Language: English; Russian
• Published: Federal Research Center of Transplantology and Artificial Organs named after V.I.Shumakov 27.01.2021
• Subjects: acute rejection; biomarkers; heart transplantation; mirna-101; mirna-27
• ISSN: 1995-1191; 2412-6160
• DOI: 10.15825/1995-1191-2020-4-20-26

Objective: to determine the diagnostic value of miRNA-101 and miRNA-27 expression levels for acute hearttransplant rejection. Materials and methods. The study enrolled 46 heart recipients, among whom were 36 men (78.3%); the average age of recipients was 47.7 = 10.8 (16 to 67) years. Serum microRNA expression levels were measured via quantitative polymerase chain reaction (PCR). Graft rejection was verified through morphological analysis of endomyocardial biopsy specimens. Results. The expression levels of miRNA-101 and miRNA-27 in recipients with acute graft rejection are significantly lower than in recipients without rejection (p = 0.04 and p = 0.03, respectively). When the miRNA-101 expression level is below the determined threshold value, the risk  of developing acute graft rejection increases 1.8 times (RR = 1.8 [95% CI 1.13–3.01]). When the miRNA-27 expression level is below the determined threshold value, the risk of developing acute graft rejection increases 1.9 times (RR = 1.9 [95% CI 1.12–3.37]). Simultaneous decrease in the expression levels of miRNA-101 and miRNA-27 below the determined threshold values increases the likelihood of acute graft rejection by 2.0 times (RR = 2.0 [95% CI 1.16–3.36]). Conclusion. The serum miRNA-101 and miRNA-27 expression levels are of diagnostic value for acute graft rejection in heart recipients.