Anticoagulant activity of heterochitosans and their oligosaccharide sulfates

• Published in: European food research & technology Vol. 219; no. 5; pp. 529 - 533
• Main Authors: PARK, Pyo-Jam, JE, Jae-Young, JUNG, Won-Kyo, AHN, Chang-Bum, KIM, Se-Kwon
• Format: Journal Article
• Language: English
• Published: Heidelberg Springer 01.10.2004; Berlin Springer Nature B.V
• Subjects: Anticoagulants; Biological and medical sciences; Chitin; Food industries; Fundamental and applied biological sciences. Psychology; Membrane reactors; Molecular weight; Sodium hydroxide; Sulfates; Sulfur; Ultrafiltration; Heterochitosan; Serine endopeptidases; Anticoagulant acitivity; Enzyme; Oligosaccharide; Thrombin; Sulfates; Thrombin time; Peptidases; Activated partial thromboplastin time; Chitooligosaccharide; Hydrolases; Sulfate
• ISSN: 1438-2377; 1438-2385
• DOI: 10.1007/s00217-004-0977-3

Three kinds of partially deacetylated heterochitosans, 90% deacetylated chitosan, 75% deacetylated chitosan and 50% deacetylated chitosan, were prepared from crab chitin by N-deacetylation with 40% sodium hydroxide solution for different durations. Nine kinds of heterochitooligosaccharides (hetero-COSs) with relatively high molecular weights (5,000-10,000 Da; 90-HMWCOSs, 75-HMWCOSs, and 50-HMWCOSs), medium molecular weights (1,000-5,000 Da; 90-MMWCOSs, 75-MMWCOSs, and 50-MMWCOSs), and low molecular weights (below 1,000 Da; 90-LMWCOSs, 75-LMWCOSs, and 50-LMWCOSs) were prepared using an ultrafiltration membrane reactor system, respectively. In addition, their sulfated derivatives were prepared by a method using a trimethylamine-sulfur trioxide, and the anticoagulant properties of the heterochitosans and their COS sulfates with different chain lengths and degrees of deacetylation were investigated. Clotting times in thrombin-time assay were prolonged in the presence of various concentrations of the heterochitosans and their COS sulfates using normal human plasma. The 90% deacetylated chitosan sulfate exhibited the highest anticoagulant activity among all the heterochitosans and their COS sulfates.[PUBLICATION ABSTRACT]