Human intestinal epithelial response(s) to Clostridium difficile

Clostridium difficile is a gram-positive, spore-forming, toxin-producing anaerobic bacillus that is being increasingly implicated as the leading cause of diarrhea and colitis, particularly in hospitalized, elderly patients. Studies to date suggest that C. difficile toxins A and B play a major role i...

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Bibliographic Details
Published inMethods in molecular biology (Clifton, N.J.) Vol. 646; p. 135
Main Authors Jafari, Nazila V, Allan, Elaine, Bajaj-Elliott, Mona
Format Journal Article
LanguageEnglish
Published United States 2010
Subjects
Bacterial Proteins - immunology
Bacterial Toxins - immunology
Caco-2 Cells
Cell Line, Tumor
Clostridium difficile - immunology
Enterotoxins - immunology
Enzyme-Linked Immunosorbent Assay
HT29 Cells
Humans
Intestinal Mucosa - immunology
Intestinal Mucosa - microbiology
Polymerase Chain Reaction
Reverse Transcriptase Polymerase Chain Reaction
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Summary:Clostridium difficile is a gram-positive, spore-forming, toxin-producing anaerobic bacillus that is being increasingly implicated as the leading cause of diarrhea and colitis, particularly in hospitalized, elderly patients. Studies to date suggest that C. difficile toxins A and B play a major role in the observed colonic inflammation and associated disease pathogenesis; however, the role of other potential bacterial factors at present remains unknown. Early effects of C. difficile on host intestinal epithelia include modest induction of innate immune responses with progressive loss of intestinal epithelial cell barrier function and cell death.
ISSN:1940-6029
DOI:10.1007/978-1-60327-365-7_9