Changes of phage T7 nucleoprotein structure at low ionic strength. A Raman spectroscopic study

To detect changes in DNA and/or protein structures of phage T7 under different ionic strength, Raman spectra of phage T7 have been recorded in solutions of three different NaCl + Tris concentrations. Iterative Jansson-Van Cittert deconvolution, as well as decomposition methods have been used to quan...

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Published inBiochimica et biophysica acta Vol. 1289; no. 1; pp. 95 - 104
Main Authors Gróf, Pál, Aslanian, Dimitrina, Rontó, Györgyi
Format Journal Article
LanguageEnglish
Published Netherlands 09.02.1996
Subjects
Bacteriophage T7 - chemistry
DNA - chemistry
Nucleic Acid Conformation
Nucleoproteins - chemistry
Osmolar Concentration
Protein Structure, Secondary
Spectrum Analysis, Raman
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Summary:To detect changes in DNA and/or protein structures of phage T7 under different ionic strength, Raman spectra of phage T7 have been recorded in solutions of three different NaCl + Tris concentrations. Iterative Jansson-Van Cittert deconvolution, as well as decomposition methods have been used to quantify changes in DNA structure. Significant modifications in ratios of contributions from 675 and 685 per cm vibrations, as well as in the DNA backbone vibrations, characteristic for B-DNA, near 835 per cm frequency have been found. Changes of the base electronic structure were identified in the interval between 1280 and 1400 cm(-1). Estimation of the overall protein structure suggests predominant beta-sheet content.
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ISSN:0304-4165
0006-3002
DOI:10.1016/0304-4165(95)00151-4