BIOCOMPATIBLE AND MATRIX PROPERTIES OF POLYLACTIDE SCAFFOLDS

• Published in: Vestnik transplantologii i iskusstvennykh organov Vol. 20; no. 2; pp. 82 - 90
• Main Authors: V. I. Sevastianov, A. M. Grigoriev, Yu. B. Basok, L. A. Kirsanova, V. N. Vasilets, A. P. Malkova, G. A. Dukhina, T. E. Grigoriev, Yu. D. Zagoskin, K. V. Tokaev, T. K. Tokaev
• Format: Journal Article
• Language: English; Russian
• Published: Federal Research Center of Transplantology and Artificial Organs named after V.I.Shumakov 27.06.2018
• Subjects: bioreactor; cytotoxicity; implantation test; mesenchymal stromal cells; polylactide scaffold
• ISSN: 1995-1191
• DOI: 10.15825/1995-1191-2018-2-82-90

Aim. A study of biocompatible and matrix properties of polylactide scaffolds as a materials for medical implanted articles as well as scaffolds for cell and tissue engineering constructions.Materials and methods. Biocompatibility of polylactide scaffolds in the form of porous disks obtained by freeze drying method was estimated in vitro: by UV spectroscopy, pH measurements and cytotoxicity to NIH/3T3 mice fi broblasts in static conditions. Biocompatibility of scaffolds in vivo was investigated by its implantation under mice skin. Matrix properties of polylactide scaffolds (cell adhesion and proliferation) were studied in dynamic conditions with mesenchymal stromal cells of human adipose tissue (MSC ADh) in perfusion bioreactor. Results. As a result of in vitro investigations it was shown that polylactide scaffolds obtained by freeze drying are satisfi ed to demands presenting biocompatible medical articles with respect to pH measurements, extraction tests and cyto toxicity to mice fi broblasts NIH/3T3. Cultivation of MSC ADh in perfusion bioreactor in hepatogenic media is accompanying by good adhesion and proliferation both on the surface and in the bulk of porous disks. However implantation of polylactide scaffolds under mice skin is accompanying by resorption and leads to the infl ammation reaction of adjacent tissues. Conclusions. Positive results obtained only by in vitro testing of biocompatibility and matrix properties are not enough to recommend the material to be used as a scaffolds for cell and tissue engineering constructions. The preliminary study of biomechanical characteristics of the implant is recommended.