Anti-birnavirus activity of methisoprinol - in vitro study with infectious pancreatic necrosis virus (IPNV)

• Published in: Acta veterinaria Brno Vol. 71; no. 4; pp. 543 - 547
• Main Authors: Siwicki, A.K. (University in Olsztyn (Poland). Dept. of Microbiology and Clinical Immunology), Morand, M, Pozet, F, Kazun, B
• Format: Journal Article
• Language: English
• Published: 01.12.2002
• Subjects: ANTIVIRAL; ANTIVIRAL AGENTS; ARN; BIRNAVIRUS; CELL CULTURE; CHEMICAL SYNTHESIS; CULTIVO DE CELULAS; CULTURE DE CELLULE; EXPERIMENTACION IN VITRO; EXPERIMENTATION IN VITRO; IN VITRO EXPERIMENTATION; INHIBICION; INHIBITION; RNA; SINTESIS QUIMICA; SYNTHESE CHIMIQUE; VIRICIDAS
• ISSN: 0001-7213; 1801-7576
• DOI: 10.2754/avb200271040543

This study was conducted to evaluate the influence of methisoprinol, synthetic anti-viral product, on the IPNV replication in vitro by measuring viral RNA synthesis. The monolayers of RTG-2 cells in tissue culture plates was cultivated with different concentrations of methisoprinol and infected with IPN virus suspension containing 10**7 TCID50/mL. At 24, 48 and 72 h after infection, the cell cultures were submitted to one hour starvation and two hours incubation with 10 microCi/mL of [3H]-uridine. Culture homogenates of each isolate were incubated with phenol/chloroform to extract RNA followed by slab polyacrylamide gel electrophoresis for 2 h. The counts were evaluated in a scintillation counter. The replicative cycle of IPN virus in RTG-2 cell culture was rapid. In control group (only infected by IPNV), the incorporation of [3H]-uridine was 45,000+/-1,500 cpm at 24 h, 186,000+/-2,450 cpm at 48 h and 554,500+/-4,550 cpm at 72 h. The percent of inhibition of IPN viral RNA labelling under methisoprinol application ranged from 5 % at 24 h to 85 % at 72 h depending on the concentration of the tested product and time when cultures were harvested. The highest percent of inhibition at 72 h after infection was observed at the dose of 1,000 microg/mL.