Two distinct blue-light responses regulate the levels of transcripts of specific nuclear-coded genes in pea

Fluence-response characteristics for bluelight(BL)-mediated changes in the steady-state levels ofCab-, pEA215 and pEA207-RNA in red-light(RL) grown pea (Pisum sativum L. cv. Alaska) seedlings indicate the existence of two BL responses: a blue-lowfluence (BLF) response, causing an increase inCab- and...

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Published inPlanta Vol. 182; no. 4; p. 553
Main Authors Warpeha, K.M.F. (Illinois Univ., Chicago (USA). Dept. of Biologial Sciences), Kaufmann, L.S
Format Journal Article
LanguageEnglish
Published Germany 01.11.1990
Subjects
ARN
CRECIMIENTO
CROISSANCE
Dunkelheit
Erbse
EXPRESION GENICA
EXPRESSION DES GENES
FITOCROMA
Genauspraegung
GENE EXPRESSION
GROWTH
Lichtfarbe
LIGHT REGIMES
NUCLEOTIDE SEQUENCE
Phytochrom
PHYTOCHROME
PHYTOCHROMES
PISUM SATIVUM
REGIME LUMINEUX
REGIMENES DE LUZ
Regulation
RNA
RNS
Saemling
SECUENCIA NUCLEICA
SEQUENCE NUCLEIQUE
Wachstum
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Summary:Fluence-response characteristics for bluelight(BL)-mediated changes in the steady-state levels ofCab-, pEA215 and pEA207-RNA in red-light(RL) grown pea (Pisum sativum L. cv. Alaska) seedlings indicate the existence of two BL responses: a blue-lowfluence (BLF) response, causing an increase inCab- and pEA215-RNA, and a blue-high-fluence (BHF) response, causing a return to control levels forCab- and pEA215-RNA and a decrease in pEA207-RNA levels (Warpeha and Kaufman, 1989, Plant Physiol.,91, 1030-1035). We now show that under dark growth conditions, only the BLF response is apparent;Cab- and pEA215-RNA increase at all fluences tested, whereas pEA207-RNA levels are unaltered over the range of BL fluence tested. The treatment of dark-grown seedlings with RL immediately prior to BHF irradiation does not elicit the BHF response forCab-, pEA215 and pEA207-RNA, indicating that the role of growth in RL is to enable the seedling to reach a particular developmental state, rather than ensuring the presence of active phytochrome at the time of BL-irradiation. The apical bud of RL-grown seedlings has only the BLF response;Cab-RNA levels increase while pEA207-RNA exhibits no change at any of the fluences tested. The developing leaves of the fourth node show the BHF response; bothCab- and pEA207-RNA decrease following treatment with high-fluence BL. These data also indicate the necessity for reaching a specific developmental state before the BHF response can be activated.
Bibliography:90G1416
F62
ISSN:0032-0935
1432-2048
DOI:10.1007/BF02341031